Suzuki T1, Lee S1, Izawa H2, Satoh A3
1Aomori University of Health and Welfare, Department of Physical Therapy, Aomori, Japan, 2Aomori University of Health and Welfare, Department of Nutrition, Aomori, Japan, 3Hirosaki University of Health and Welfare, Department of Nursing, Hirosaki, Japan
Background: Psychogenic stress has negative effects on various disease states related to oxidative stress, and exercise is frequently used as a remedy for improvement. However, the impact of the nature of various exercise activities on oxidative stress and antioxidation capacity while under the influence of psychogenic stressors is unclear, and clarification of the relationships between these aspects is needed.
Purpose: In this study, we aimed to clarify the effect of different types of exercise activities on the dynamics of oxidative stress and antioxidation capacity while under the influence of psychogenic stressors and, additionally, to develop a risk management approach for exercise load and the expertise necessary for preventive medicine.
Methods: Twenty-four 8-week-old Wistar male rats were used as test animals and were placed into a control group (C group), a psychogenic stress (PS group), a PS-aerobic group, or a PS-anaerobic group. The small Island stress (SIS) method was used to produce psychogenic stress, and the three groups other than the C group were subjected to psychogenic stress for 1 week. In addition, MK-680 was used for the exercise load and was performed at the same time once daily from the start of the experiment. The exercise load used for the PS-aerobic group called for a slope of 0%, a speed of 20.0 m/min, and an exercise time of 60 minutes. In the PS-anaerobic group, the exercise load called for a slope of 0%, a speed of 45.0 m/min, and an exercise time of 10 minutes. Diacron reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) were measured both before and after the experiment. For statistical analysis, a paired t-test was performed, and the level of statistical significance was set at p 0.05.
Results: The d-ROM value, an index of the oxidative stress level, significantly increased by 32.0 % in the PS group after the experiment compared with pre-experiment levels (p 0.01). Additionally, d-ROM values significantly increased by 43.6 % and 56.3 % in the PS-aerobic and PS-anaerobic groups, respectively (p 0.001 for both). In contrast, BAP, an index of antioxidative capacity, significantly decreased (by 12.3 %) after the experiment only in the PS-aerobic group. e (p 0.001).
Conclusion(s): The responses to different exercise loads in a psychogenic stress environment suggested the possibility that the state of hyperoxidative stress can differ depending on exercise intensity and on when antioxidative capacity is limited. In essence, a new exercise load at the time of the onset of psychogenic stress can lead to exacerbation of oxidative stress and may be a new physiological risk factor. Therefore, rest or light physical activity may be desirable when psychogenic stressors appear, as oxidative stress production is suppressed during this time.
Implications: The results of this study can be used not only for supporting rehabilitative therapies in cases of mental illness but also for deciding exercise loads for patients experiencing psychogenic stress.
Keywords: Psychotogenic stress, Exercise, Oxidative stress
Funding acknowledgements: The present work was supported by the Aomori University of Health and Welfare (AUHW).
Purpose: In this study, we aimed to clarify the effect of different types of exercise activities on the dynamics of oxidative stress and antioxidation capacity while under the influence of psychogenic stressors and, additionally, to develop a risk management approach for exercise load and the expertise necessary for preventive medicine.
Methods: Twenty-four 8-week-old Wistar male rats were used as test animals and were placed into a control group (C group), a psychogenic stress (PS group), a PS-aerobic group, or a PS-anaerobic group. The small Island stress (SIS) method was used to produce psychogenic stress, and the three groups other than the C group were subjected to psychogenic stress for 1 week. In addition, MK-680 was used for the exercise load and was performed at the same time once daily from the start of the experiment. The exercise load used for the PS-aerobic group called for a slope of 0%, a speed of 20.0 m/min, and an exercise time of 60 minutes. In the PS-anaerobic group, the exercise load called for a slope of 0%, a speed of 45.0 m/min, and an exercise time of 10 minutes. Diacron reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) were measured both before and after the experiment. For statistical analysis, a paired t-test was performed, and the level of statistical significance was set at p 0.05.
Results: The d-ROM value, an index of the oxidative stress level, significantly increased by 32.0 % in the PS group after the experiment compared with pre-experiment levels (p 0.01). Additionally, d-ROM values significantly increased by 43.6 % and 56.3 % in the PS-aerobic and PS-anaerobic groups, respectively (p 0.001 for both). In contrast, BAP, an index of antioxidative capacity, significantly decreased (by 12.3 %) after the experiment only in the PS-aerobic group. e (p 0.001).
Conclusion(s): The responses to different exercise loads in a psychogenic stress environment suggested the possibility that the state of hyperoxidative stress can differ depending on exercise intensity and on when antioxidative capacity is limited. In essence, a new exercise load at the time of the onset of psychogenic stress can lead to exacerbation of oxidative stress and may be a new physiological risk factor. Therefore, rest or light physical activity may be desirable when psychogenic stressors appear, as oxidative stress production is suppressed during this time.
Implications: The results of this study can be used not only for supporting rehabilitative therapies in cases of mental illness but also for deciding exercise loads for patients experiencing psychogenic stress.
Keywords: Psychotogenic stress, Exercise, Oxidative stress
Funding acknowledgements: The present work was supported by the Aomori University of Health and Welfare (AUHW).
Topic: Professional practice: other
Ethics approval required: Yes
Institution: Aomori University of Health and Welfare
Ethics committee: Research Ethics Committee
Ethics number: No.18002
All authors, affiliations and abstracts have been published as submitted.
