Martignago C.1, Tim C.2, Bossini P.3, Neves L.4, Liebano R.1, Parizotto N.A.1
1Federal University of São Carlos, Physiotherapy, São Carlos, Brazil, 2Federal University of São Paulo, Bioscience, Santos, Brazil, 3University Sacred Heart, Phsiotherapy, Bauru, Brazil, 4Federal University of São Carlos, Physiology, São Carlos, Brazil
Background: The skin flap is a technique often used in reconstructive plastic surgery or loss of tissue, which presents the main complication of tissue ischemia that may progress to necrosis of the flap. Research indicates that low level laser therapy (LLLT) may be used as an effective therapeutic approach to reduce the area of necrosis, however, there is divergence of power to be used.
Purpose: To assess the effects of the application of LLLT (660 nm) with different powers on the viability of random skin flap in rats.
Methods: For this study we used 18 (Rattus norvegicus: var albinos, Rodentia Mammalia.), male Wistar were randomly divided into 3 groups (n = 6); GC: subjected to dummy treatment of LLLT, the G50: subjected to irradiation with LLLT with a fluency of 220 J / cm2 and power of 50 mW for 120 seconds and the G100 subjected to irradiation with LLLT with a fluency 220 J / cm2 and power of 100 mW for 60 seconds, irradiated in both groups was given an energy of 6 J per point. The animals were irradiated with a laser Indio, Gallium, aluminum and phosphorus (InGaAlP), 660 nm (Photon Lase III, DMC®, São Carlos, SP, Brazil) with 0.028 cm2 beam area in continuous emission mode applied 3 points in the cranial base of the flap. Treatment began immediately after completion of the random skin flap of cranial base (dimension 10X4cm2) being reapplied every 24 hours to complete 5 applications. The percentage of necrotic area was evaluated on the 7th postoperative day with the method of feedback and paper. For statistical analysis, there was the ANOVA One Wey-test and post-hoc Tukey.
Results: There was a statistically significant difference (p 0.001) between the control group (64.38% ± 7.5) and G50 (37.66% ± 12.96) in the G50 animals showed better results compared to animals GC, there was also a statistically significant difference (p = 0.003) between the G50 and the G100 (59.87% ± 6.14) where animals of G50 demonstrated better results in relation to the reduction of necrosis area. There was no significant difference (p = 0.688) between the animals of CG and G100.
Conclusion(s): There are differences between the power LLLT application to increase the flap viability.
Implications: The results of this research suggest that LLLT can be used in clinical practice for preventing skin flap necrosis formation, a common complication in the reconstructive plastic surgery or tissue loss, and shortens the length of stay and spending on hospitalization of patients.
Funding acknowledgements: We would like to acknowledge the contributions of Brazilian funding agency Fapesp (FAPESP project 2015/13501-3) for the financial support
Topic: Electrophysical & isothermal agents
Ethics approval: The experiment was approved by the Ethics Committee on Animal Experiments of UFSCar by Protocol 6,224,290,515 day 15.07.2015.
All authors, affiliations and abstracts have been published as submitted.
