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Muniz Renno A.C.1, Yamauchi L.2, Harue Moriyama J.2, Mituyama L.2, Galdino Janeiro J.2, Assis L.2, Kido H.2, Máximo Prado C.2
1University of Sao Paulo, Biosciences, Santos, Brazil, 2University of Sao Paulo, Santos, Brazil
Background: Sepsis is defined as an infection with secondary systemic disorders and respiratory complications are particularly important because of the severity, frequency and considerable impact on public health.
Polymicrobial sepsis induced by cecal ligation and puncture (CLP) is the gold standard to sepsis research, because it closely resembles the progression and characteristics of human sepsis.
The low-level laser therapy (LLLT - Low-Level Laser Therapy) has often been used as a therapeutic strategy noninvasive, safe and effective aimed to modulate the inflammatory response in several pathological conditions. However, the performance of LLLT on lung tissue during sepsis remains uncertain.
Polymicrobial sepsis induced by cecal ligation and puncture (CLP) is the gold standard to sepsis research, because it closely resembles the progression and characteristics of human sepsis.
The low-level laser therapy (LLLT - Low-Level Laser Therapy) has often been used as a therapeutic strategy noninvasive, safe and effective aimed to modulate the inflammatory response in several pathological conditions. However, the performance of LLLT on lung tissue during sepsis remains uncertain.
Purpose: The aim of this study was to evaluate and compare the effects of LLLT on acute lung inflammation resulting from a polymicrobial sepsis model (cecal ligation and puncture- CLP).
Methods: Federal University of São Paulos Ethical Committee (Number 8948050515) approved this study. Sixteen male Wistar rats, ±300 g, 3 months old were randomly divided into 2 groups: sepsis group (S): rats submitted to CLP without LLLT treatment and sepsis laser treated group (SL). Laser irradiation (AlGaAs, continuous wave, 808 nm, 30 mW, 28 s, 30 J/cm2; 0.028 cm2; 0.8 J, PHOTON LASER II, DMC® equipment Ltda, SP, São Carlos, Brazil) was performed immediately after surgery on the anterior portion of the trachea near the carina and anterior portion of the chest near the diaphragm bilaterally, through punctual contact technique. Bronchoalveolar lavage technique was performed as follow: instillation of 3 ml of saline into the trachea (3 times), centrifugation at 1,000 rpm for 10 minutes at 4 ° C, the cell pellet was re-suspended with 1 ml of PBS to count the total number of cells per ml carried out in a Neubauer chamber.Histological and immunohistochemical analysis are in progress. Animals were euthanized 24 hours post-surgery.
Results: The treated group showed a decrease in the number of inflammatory cells in both bronchoalveolar lavage fluid (BAL) and in histological analysis of lung tissue.
Conclusion(s): These results suggest that LLLT could be an effective therapeutic approach modulating inflammatory response in acute lung injury caused by sepsis. However further analysis is needed to clarify the effects of LLLT on this model of sepsis.
Implications: By knowing the mechanisms which laser therapy modulates the process of pulmonary inflammation using the sepsis model that most closely resembles to human sepsis , we expect this study to contribute to the understanding of an effective therapy, such as LLLT, for the treatment of patients with acute lung injury. As a result, the reduction in the number of days of hospitalization may be expected as well as improving life quality of sepsis patients.
Funding acknowledgements: National Counsel of Technological and Scientific Development- CNPq
Topic: Disability & rehabilitation
Ethics approval: Federal University of São Paulos Ethical Committee (Number 8948050515) approved this study.
All authors, affiliations and abstracts have been published as submitted.
